PLEASE NOTE: When ordering this item, please select FedEx Overnight as the shipping. This is required due to the temperature-sensitive nature of some components. All orders received with the incorrect shipping item will be canceled.
Nate’s Plates is a PCR-based normalization kit designed to incorporate dual indexing tags and return equal numbers of sequencing constructs from each sample.
This kit replaces PCR2 in the standard GT-seq library preparation protocol and eliminates the need for expensive plate-based amplicon capture/normalization.
This process saves time by combining tagging and normalization into a single step and saves money by omitting pipetting steps coupled with 5μL reaction volumes.
Nate’s Plates are designed to work with the following Illumina tag sequences:
- R1: CTACACGTTCAGAGTTCTACAGTCCGACGA
TC OR CGACTCGTTCAGAGTTCTACAGTCCGACGA TC
- R2: GTGACTGGAGTTCAGACGTGTGCTCTTCCG
This Kit includes:
- 105 unique 96-well tagging plates
- Magnetic Beads
- Wash Buffer
- Bead Release Primer
- Bead Buffer
User-Supplied Reagents Not Included:
- PCR Master Mix (we recommend QIAGEN Multiplex PCR Kit)
- Magnetic Stand/Rack
- Nuclease-free H₂O
- Optional Centrifuge Pooling Trays (GTseek SKU #2001)